Characterization of Escherichia coli from diarrheic calves with special reference to plasmid profile

Out of 120 fecal samples collected from diarrheic calves at different localities in AL-Qalyoubia Governorate. 43 were positive for Escherichia coli with an incidence of 35.83%.Serogrouping of E.coli isolates recovered 8 belong to different "O" serogroups which were O55, O111, O26, O153, O8, O18, O86, O157, and "5" isolates were untypable. Antibiogram pattern of isolated E.coli serogroups showed that all tested serogroups of E.coli were sensitive to enrofloxacin, and neomycin, all groups were resistant to cefadroxil, colistin sulphate, Tobramicin and ampicillin .meanwhile variable results were recorded with the remaining used chemotherapeutic agents. Plasmid profile analysis of various E.coli serogroups revealed that E.coli serogroups O111 revealed 4 plasmids with their super coiled forms with a molecular weightranging1-7 Kbp, while E.coli serogroup O153 revealed 3 plasmid with molecular weight 2.5-7 Kbp and O18 harbored 2 plasmid with molecular weight 1.8-2.5 Kbp, while E.coli serogroups O26, O55, O8 revealed 1 plasmid with the same molecular weight 7 Kbp. On the other hand E.coli serogroups O86 have no plasmid. [El-Shehedi Mona, A., Mostafa, M. Eraqi, and Aisha, R. Ali. Characterization of Escherichia coli from diarrheic calves with special reference to plasmid profile. J Am Sci 2013;9(7):54-59]. (ISSN: 1545-1003). http://www.jofamericanscience.org. 6